FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859398
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT424862.284933080491643No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA334821.8006903309565785No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG332771.7896652572499272No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT263081.4148665320711327No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG21820.11734980891664937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT63300.0216.002328
GCAGGTC60500.0178.946462
AGTCTTT82550.0165.810999
CAGGTCA67450.0160.289263
GCCATAC87200.0157.983891
GTCAATT69700.0153.63366
AGGCTGT87250.0151.976585
ATTAGGC90200.0147.33322
AATTTCA78850.0135.992549
GGCAGGT85700.0128.048511
TTAGGCT107400.0124.287283
GCTGTGG109500.0120.9607857
GTCCATG1350.0120.182524
ATCTTAC67200.0120.0605853
ACCCCGC71350.0112.663948
CTTACCC71600.0112.270555
GGTCAAT95650.0112.1067665
AGGTCAA96150.0111.677194
TCAATTT99500.0108.2136847
TCTTACC75200.0107.288184
Produced by FastQC (version 0.11.5)