FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859398
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT384572.0682500465204328No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA314461.691192525752959No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG310691.6709171463021903No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT240681.2943974340082112No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG19210.10331300775842504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT67850.0198.047828
GCAGGTC62350.0172.675032
AGTCTTT81200.0164.578729
CAGGTCA66200.0163.746663
GCCATAC82850.0159.494951
ATTAGGC87750.0156.81142
AGGCTGT86700.0156.511735
GTCAATT70350.0153.68856
AATTTCA76450.0143.933849
TTAGGCT101000.0136.385623
GCTGTGG107900.0125.220537
GGCAGGT87450.0123.616261
ATCTTAC66900.0123.013053
AGGTCAA89650.0120.427954
GGTCAAT91850.0118.349525
ACCCCGC69400.0115.622128
CTGTGGG118050.0114.0791558
TCAATTT97250.0112.997347
TAGGCTG122500.0111.9734
TCTTACC74350.0110.097774
Produced by FastQC (version 0.11.5)