FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329448
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT75852.3023360287511228No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG66612.021866880357446No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA39581.201403559894126No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT36311.1021466210145456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT9900.0244.343438
TCTCGTT850.0208.2353295
GCAGGTC10400.0204.230772
AGTCTTT13800.0175.289869
GCCATAC14250.0169.754381
GTCAATT11950.0166.631796
CAGGTCA12600.0166.230163
GGCACTT200.00673553147.5295
TCAATGG503.346213E-8147.57
GTCCCCC200.00673553147.5295
AATTTCA13900.0144.316549
ATTAGGC12300.0143.902452
GGCAGGT15950.0134.090911
GGTCAAT16650.0124.9099045
TCAATTT16200.0122.916687
CACACGC854.7293724E-11121.4706295
AGGCTGT14550.0120.635745
AGGTCAA17450.0118.3381044
CTAGTCT20650.0117.142867
CCGCTCC1450.0111.89655295
Produced by FastQC (version 0.11.5)