FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619138
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT89121.4394206138211514No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA81331.3136005220160933No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT76631.237688528244107No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG74031.195694659348966No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG14960.24162626102742843No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT9010.1455249072097012No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG6660.10756891032370813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG150.0049664127163.333337
TAGTCGT150.0049664127163.333338
GAAGGTC402.6788257E-8153.1252
CGTACCC251.1644695E-4147.05
TCGTACC251.1644695E-4147.04
TTACCCG251.1644695E-4147.06
AGGCTGT23750.0145.968435
ATCTTAC15250.0136.557373
ATTAGGC28500.0121.640352
TAGTCTT16750.0121.402998
GTCAATT17050.0120.703816
TTAGGCT29150.0118.9279563
CTTACCC17550.0118.6609655
GCAGGTC18650.0112.9758762
GCTGTGG30350.0112.6112067
CAGGTCA18700.0111.363633
AACTCCC2800.0109.37501245
TCAATGT458.8508805E-6108.888897
AGTCTTT20500.098.597569
CTGTGGG34250.096.927018
Produced by FastQC (version 0.11.5)