FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619138
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA87101.406794607987234No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT86291.3937119026775937No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG80311.2971260042187687No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT68731.110091772755024No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG11260.18186575529203505No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG6580.1062767912807807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCC100.0014760375245.00002245
CGTACCC354.9112714E-11210.000025
GTACCCC304.802132E-9204.166666
TCGTACC600.0204.166664
CGTACCT254.7481626E-7196.05
GGCTGTA1300.0188.461536
TACCTCG452.8376235E-10163.333347
GCGTCGG1300.0141.34616245
AGGCTGT27550.0140.508165
TAGTCTT13700.0136.806588
ATCTTAC15900.0125.581763
ATTAGGC33100.0125.4607242
GTACCTC404.9283244E-6122.500016
CGGATTG501.0148142E-7122.5245
CAGGTCA17450.0121.4473
GTCAATT17500.0119.76
TTAGGCT34400.0117.514533
GCAGGTC18950.0113.1266562
CTTACCC17550.0113.076925
AGTCTTT16750.0111.895539
Produced by FastQC (version 0.11.5)