FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667689
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG83791.2549255716358965No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT80701.208646540530097No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT77431.1596716435346397No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA67111.0051086658609023No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG12510.18736267933124554No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG7110.10648670264149926No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTC100.0014760636244.999986
GAAGGTC351.2065357E-8175.02
ATACGAG150.0049664984163.333334
TAGTCTT16100.0133.152188
AGGCTGT28200.0123.803195
CGTACCC404.92861E-6122.499995
TCGTACC404.92861E-6122.499994
ATTAGGC30450.0117.068972
ATCTTAC19200.0113.567713
GCAGGTC20450.0112.6161352
GTCAATT20000.0111.4756
TTAGGCT32450.0107.9660953
CAGGTCA21350.0107.868863
AGTCTTT20250.0107.6790169
CGACTTT852.055458E-10100.882355245
GCCATAC23200.099.267241
CTTACCC22000.098.5568165
GTACCCC501.4939074E-598.06
GCTGTGG35700.096.078437
GGGTTAT16700.091.69162245
Produced by FastQC (version 0.11.5)