FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667689
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG95551.4310554764268992No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT80771.2096949328205198No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT72561.0867334941866649No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA68021.0187377656363965No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG7890.1181687881633515No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG7620.11412498932886418No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6840.10244290380701195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCT254.7484536E-7196.05
TCGTACC401.2551027E-10183.749984
TCGTAAT150.0049664984163.333335
ACGTACG150.0049664984163.33333245
TTACCCG150.0049664984163.333336
TACCTCG352.5365316E-6140.07
GTCAATT20100.0128.594536
GCAGGTC20850.0126.906472
GGCTGTA1500.0122.56
TAGTCTT17000.0122.499998
ATCTTAC18950.0120.2374653
CAGGTCA22000.0119.715913
AGGCTGT28600.0116.075175
GTACCTC551.7916864E-7111.363646
CTTACCC20700.0111.2560355
ATTAGGC32350.0110.193192
AATTTCA24150.0108.043479
ACCCCGC21400.0107.0443958
TTAGGCT32800.0106.440553
TCTTACC22250.0103.505624
Produced by FastQC (version 0.11.5)