FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534020
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT62431.1690573386764542No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG60861.139657690723194No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT60831.1390959140107113No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA57361.0741170742668813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC201.560984E-7245.000024
CGTACCC301.1773391E-6163.333335
TAGTCTT13050.0122.969358
GTACCCC404.927697E-6122.500016
GCAGGTC12750.0108.568632
CAGGTCA13100.0106.603053
GTCAATT12950.0105.06
AGGCTGT16300.0104.463195
AGTCTTT16550.097.703939
ATCTTAC13200.096.515153
ATTAGGC18650.093.2707752
CTTACCC14050.089.804275
GCCATAC18550.085.849061
TTAGGCT20200.085.507433
CCGTTTT450.001206479681.66667245
GGGTTAT13850.081.37184245
TGAGCAT7150.077.0979245
GCTGTGG22400.076.015627
TCAATGT500.001832881273.499997
AATTTCA18900.072.59269
Produced by FastQC (version 0.11.5)