FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534020
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG64931.2158720647166772No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA61991.1608179468933748No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT60051.1244897194861614No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT59381.1119433729073818No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCT201.560984E-7245.000025
TACCTCG251.6134436E-9244.999987
TCGTACC304.800313E-9204.166664
GGCTGTA850.0144.117656
GTACCTC352.5360623E-6139.999986
TAGTCTT11000.0125.840918
GTCAATT14500.0118.2758566
AGGCTGT18900.0116.666675
GCAGGTC14850.0114.66332
CAGGTCA15200.0112.0230263
ATCTTAC12800.0111.015633
CTTACCC14700.099.1666645
AGTCTTT14450.096.64369
ATTAGGC23400.096.324792
TTAGGCT23750.094.389473
AATTTCA18900.093.981489
GGGTTAT15450.090.38835245
TCTTACC16500.087.606064
ACCCCGC17800.083.960678
AGGTCAA20500.083.658534
Produced by FastQC (version 0.11.5)