FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343951
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT78552.2837555349453846No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT76022.210198545723083No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG75932.207581893932566No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA45121.3118147643123583No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT6400.18607301621451894No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG6170.17938601719430963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)