FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349789
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT75402.155585224235182No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG68901.9697589118011143No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT53591.5320664743602572No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA45281.2944946810791649No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGT202.2807664E-5221.255
TAGTCTT10350.0210.917888
CCGGTCC304.665635E-7196.66667295
TGCCGTT150.0028488527196.66667295
GCAGGTC11750.0193.319152
TCTCGTT900.0163.8889295
GTCAATT13650.0161.007326
CAGGTCA14350.0159.320563
ATTAGGC12900.0152.073642
GGCAGGT15350.0148.941381
GCCATAC14750.0148.000021
AGTCTTT14950.0147.006689
AGGCTGT13400.0144.197755
AATTTCA15950.0139.63959
ATCTTAC14250.0123.1754463
AGGTCAA19050.0119.238844
GGTCAAT19250.0115.70135
TCAATTT19400.0114.0463947
TCTTACC15350.0113.387634
ACCCCGC15350.0113.387638
Produced by FastQC (version 0.11.5)