FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349789
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG76842.1967528996051904No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT60041.7164633536217548No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA50631.447444030544128No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT47141.3476695950987594No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG3720.10634982803918935No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT8650.0206.329488
GCAGGTC13450.0186.431212
CAGGTCA14900.0167.298663
AATTTCA15150.0161.617169
GTCAATT15500.0157.967746
GCCATAC12200.0147.51
CGACTTT200.0067357067147.5295
AGTCTTT12350.0144.514189
GGCAGGT17800.0141.699451
ATTAGGC13950.0135.34052
AGTCCCC453.5130997E-6131.11111295
AGGTCAA19050.0129.304464
GGTCAAT19850.0123.350125
AGGCTGT15550.0120.466245
TCAATTT21000.0115.892857
ATCTTAC12550.0104.601593
ACCCCGC12300.0104.329278
CTAGTCT17650.0101.118997
TCTTACC12850.0101.011674
TTAGGCT19300.098.588093
Produced by FastQC (version 0.11.5)