FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532158
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT345062.2521176014484148No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG331242.1619180267309246No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT298311.9469924120097275No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA294451.9217991878122231No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG17030.11115041660194314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT48050.0213.033088
GCAGGTC58100.0180.498842
CAGGTCA59250.0177.244453
GTCAATT62750.0167.82846
AGGCTGT66900.0165.795495
AGTCTTT62050.0165.680739
ATCTTAC59050.0151.11813
ATTAGGC73600.0151.103492
GCCATAC68100.0150.311871
AATTTCA72000.0146.676829
CTTACCC63150.0141.30685
GGCAGGT77500.0136.838461
ACCCCGC66400.0134.834678
TACCCCG69200.0129.805227
TTAGGCT86500.0128.568973
GCTGTGG87550.0126.6900947
TCTTACC70800.0126.455114
AGGTCAA83550.0126.047064
GGTCAAT84650.0124.409125
TCAATTT86300.0122.543237
Produced by FastQC (version 0.11.5)