FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1532158
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT336532.1964444920171418No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG331152.161330619949118No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA302541.974600530754661No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT299251.9531275495086016No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG17350.11323897404836838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT50200.0203.052418
GCAGGTC57050.0181.510222
CAGGTCA59300.0175.369523
GTCAATT61850.0168.865746
AGTCTTT61850.0164.805689
ATTAGGC71750.0159.947462
ATCTTAC58450.0158.487413
AGGCTGT73050.0154.687975
AATTTCA67850.0154.36279
GCCATAC65950.0154.08751
CTTACCC65200.0141.862635
ACCCCGC66750.0139.00598
TTAGGCT85800.0133.92753
GGCAGGT79400.0131.700881
TCTTACC70450.0131.500264
TACCCCG71050.0130.389797
GCTGTGG88250.0126.707557
GGTCAAT83150.0125.0762565
CCCCGCC74500.0124.941569
AGGTCAA83150.0124.898844
Produced by FastQC (version 0.11.5)