FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547288
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT349292.2574336516537326No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG321602.07847537110092No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA299361.9347400096168266No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT293101.8942821245947747No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG19650.1269963962752894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT52250.0213.972238
GCAGGTC58050.0181.413882
CAGGTCA61800.0170.405763
GTCAATT62650.0168.564626
AGTCTTT68850.0162.596929
AGGCTGT70300.0161.76095
GCCATAC69900.0160.787521
ATTAGGC74250.0153.354082
GTCACGG900.0147.49405295
AATTTCA72350.0147.188269
ATCTTAC59900.0139.614583
GGCAGGT79400.0133.747761
CTTACCC64550.0129.785635
GCTGTGG87450.0129.700327
TTAGGCT88550.0129.088523
ACCCCGC66300.0125.914998
AGGTCAA84200.0125.422494
GGTCAAT84900.0124.040945
TCAATTT86650.0123.57847
TCTTACC68700.0122.589674
Produced by FastQC (version 0.11.5)