FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366678
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG76472.085480994223815No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT71661.954303230627417No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA52111.421137892101517No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT39331.0726032104462224No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT9950.0222.36188
GCAGGTC12750.0195.509812
GTCAATT14350.0172.682926
CAGGTCA15050.0164.651153
AATTTCA15750.0156.39689
ATTAGGC13700.0156.113132
GCCATAC14350.0154.181181
AGTCTTT14450.0153.114189
AGGCTGT15550.0136.591635
GGCAGGT18550.0134.380051
GGTCAAT19900.0124.522615
TCAATTT19900.0124.522617
AGGTCAA20000.0123.16254
ATCTTAC11800.0112.53
CTAGTCT19700.0112.309657
TTAGGCT19250.0111.870133
ACCCCGC11900.0107.836138
TACCCCG12050.0106.493787
TCTTACC12700.0105.688974
CAATTTC23700.0103.934598
Produced by FastQC (version 0.11.5)