FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423134
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT90132.1300580903449027No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG89602.1175325074326334No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT57431.357253257833216No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA56941.3456730019331937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT11450.0217.707418
TCTCGTT850.0208.23529295
GCAGGTC13900.0205.86332
GTCACGG1050.0168.57144295
GGTGCCA351.005832E-6168.571431
CAGGTCA17200.0167.223833
GTCAATT17450.0163.137546
AATTTCA18850.0151.021229
AGTCTTT16700.0149.266469
GGCGGTC200.006736202147.52
GGCAGGT20100.0142.363171
GCCATAC17850.0140.476181
ATTAGGC16300.0133.021472
GATTCCC1350.0131.1111295
AGGCTGT17500.0123.8999945
AGGTCAA23350.0123.811564
GGTCAAT23200.0122.704745
TCAATTT24650.0116.683577
CTAGTCT23650.0105.401697
ATCTTAC16350.0100.137623
Produced by FastQC (version 0.11.5)