FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489072
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT307172.062828392448451No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT292211.9623631362351852No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG275121.8475936690771164No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA218341.4662823557222215No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG70780.4753296012550099No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT25460.17097897213835192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT45700.0210.755788
GCGGTCG603.6379788E-12172.079563
ATTAGGC75150.0167.221882
AGGCTGT75100.0166.154825
AGTCTTT59150.0163.331159
GCAGGTC56000.0159.08582
GGCGGTC655.456968E-12158.842672
GCCATAC62650.0154.441941
ATCTTAC63900.0151.189943
GTCAATT61200.0145.809716
CAGGTCA61500.0145.338263
ACCCCGC66350.0145.16268
CTTACCC67450.0142.576555
TTAGGCT89600.0139.924383
TCTTACC70350.0137.118524
TACCCCG72550.0132.553947
GCTGTGG94400.0131.247127
CCCCGCC73900.0129.932869
AATTTCA72100.0124.584639
AAATCTT80400.0120.712521
Produced by FastQC (version 0.11.5)