FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489072
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT299152.008969344665671No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG287751.9324115959470058No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT285711.9187117882815605No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA229681.5424371689213148No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG67800.4553171371162711No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT25430.17077750437856598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT46250.0200.288168
GCAGGTC56900.0171.061222
ATTAGGC74600.0169.61662
AGGCTGT74150.0168.469515
ATCTTAC62850.0160.02913
AGTCTTT59150.0157.106169
ACCCCGC64600.0153.898648
CAGGTCA63850.0152.210423
GTCAATT64850.0150.343376
TCTTACC66900.0150.130874
CTTACCC66700.0149.91775
GCCATAC62550.0148.998021
TTAGGCT86200.0147.133333
TACCCCG70700.0140.82897
GCTGTGG90300.0137.214167
AATTTCA71100.0136.717229
CCCCGCC73150.0135.708839
TTACCCC78600.0127.0453956
AAATCTT79000.0126.5590741
GGCAGGT78300.0123.9238361
Produced by FastQC (version 0.11.5)