FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645291
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG82401.2769432705554549No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT81231.2588119158643154No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT80781.251838317906185No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA70921.0990390382013695No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG15570.2412864893513159No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG9060.14040177222369443No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG7680.11901607181876084No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT6920.10723843971169596No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAC150.0049664597163.333331
CGTACCC301.1775264E-6163.333335
TCGTACC301.1775264E-6163.333334
TTACCCG150.0049664597163.333336
TAGTCTT16800.0131.258
GCAGGTC19500.0128.153852
AGGCTGT27850.0127.11855
CAGGTCA21000.0120.1666643
ATTAGGC31950.0116.940542
GTCAATT20800.0116.610586
CTTACCC18000.0111.611115
ATCTTAC17800.0111.488763
TTAGGCT33950.0106.804123
TGAGCAT9300.0105.37635245
AGTCTTT22150.098.995489
GCCGTGG501.4938694E-598.07
AATTTCA26000.094.701919
GCTGTGG36250.094.620697
GCCATAC24250.091.938151
ACCCCGC22400.091.3281258
Produced by FastQC (version 0.11.5)