FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645291
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG90991.4100615071339906No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT77131.1952746900235707No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT75521.170324706217815No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA70751.0964045678616314No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG10540.16333716106376814No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG9700.15031977820859116No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG8130.12598966977689136No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG7500.11622663263550863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTCG304.802132E-9204.166677
GGCTGTA1100.0178.181826
TCGTTGC150.0049664597163.333333
CGTTGCG150.0049664597163.333334
TCGTACC301.1775264E-6163.333334
AGTCGTT150.0049664597163.333331
CGTACCC251.164492E-4147.05
AGGCTGT29100.0130.077325
ATCTTAC17650.0126.317283
ATTAGGC33850.0123.042832
TCAATGG404.9284827E-6122.500017
GCAGGTC19650.0122.18832
CAGGTCA19850.0120.957183
GTCAATT19250.0120.9090966
TTAGGCT35550.0116.125183
TAGTCTT15850.0115.157748
GTACCCC354.4444262E-4105.06
CTTACCC20600.0104.065535
AATTTCA22350.0103.59069
TCTTACC21650.0101.281754
Produced by FastQC (version 0.11.5)