FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334336
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT430543.2266235790685402No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT385482.888927526500072No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA316332.3706922394359444No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG268582.0128363470670054No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT95380.714812461029306No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG68710.5149377667993669No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT55360.41488800422082595No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA15900.11916039138567798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT52850.0216.848928
AGGCTGT79950.0206.808525
ATCTTAC65400.0195.534153
ATTAGGC86250.0192.899552
CTTACCC68400.0186.742455
AGTCTTT62700.0182.782569
ACCCCGC71950.0178.14368
GCTGTGG95100.0173.862677
TTAGGCT95750.0173.298583
GCCATAC67000.0172.152471
TCTTACC74400.0171.880834
CCCCGCC76400.0167.767449
TACCCCG77100.0165.861657
AAATCTT81700.0157.24521
TTACCCC82350.0155.108496
TAGGCTG112000.0148.154824
GGCTGTG112050.0147.825426
GCAGGTC68250.0146.739962
CAGGTCA69100.0145.148353
GTCAATT69950.0142.962866
Produced by FastQC (version 0.11.5)