FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334336
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT425703.1903508561561704No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT371492.7840813708091514No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA331842.486929828768766No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG277262.077887428653652No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT81740.612589332821718No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG73360.5497865605064991No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT39980.2996246822389563No Hit
GGCAGGTCAATTTCAGTGGTAGTAATATAATTGTTGGGACGATTAGTTTT16510.12373195357091467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGT80250.0214.502095
TAGTCTT55600.0209.599668
ATCTTAC64250.0207.524053
ATTAGGC86200.0200.186812
CTTACCC68300.0193.721335
AAGGTCC1300.0192.82564295
TTAGGCT92000.0187.252733
AGTCTTT62650.0186.24899
ACCCCGC73300.0181.526848
TCTTACC75150.0177.430764
GCTGTGG95350.0177.297337
TACCCCG77950.0170.319617
GCCATAC69250.0168.253521
CCCCGCC79350.0166.570989
AAATCTT81150.0164.844961
TTACCCC81800.0162.664026
TAGGCTG109400.0157.611044
GCAGGTC68000.0153.344212
CAGGTCA68350.0152.780493
GTCAATT68850.0151.907966
Produced by FastQC (version 0.11.5)