FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516785
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT504913.3288172021743363No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT481663.1755324584565376No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG303131.998503413469938No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA295481.9480677881176305No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT126160.8317592803198871No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT122320.8064425742606897No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG52350.3451379068226545No Hit
GGCAGGTCAATTTCAGTGGTAGTAATATAATTGTTGGGACGATTAGTTTT45100.2973394383515132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)