FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370669
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG85612.309607763260483No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT78922.1291232878929716No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA53841.4525088421205983No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT46431.2526000285969423No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT11150.0242.08528
GCAGGTC15050.0215.614642
CAGGTCA16450.0194.574483
GTCAATT16100.0193.307456
AATTTCA18000.0172.902779
GCCATAC16150.0169.876161
AGTCTTT16450.0164.088159
GGCAGGT21200.0155.849061
GGTCAAT21200.0148.195775
CCGGTCC401.9551844E-6147.5295
AGGTCAA21200.0146.804264
ATTAGGC14700.0140.47622
TCAATTT22750.0136.802197
GTCCATG453.5133799E-6131.111114
CTAGTCT22050.0122.414967
AGGCTGT16950.0120.95875
AAGGTCC505.9331833E-6118.0295
CCATACT23500.0118.02
CAATTTC27500.0114.7818158
TCTTACC13150.0114.4106454
Produced by FastQC (version 0.11.5)