FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595883
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT352372.2079939444182313No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT305651.915240653606812No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG291551.8268883119877837No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA220571.3821188646034828No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG95600.5990414084240512No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT38990.2443161560089305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT45700.0214.306478
ATTAGGC78400.0168.756232
AGGCTGT78400.0168.568085
AGTCTTT61500.0160.20829
ATCTTAC66500.0152.598483
GCAGGTC61450.0150.257392
GCCATAC65950.0149.39811
ACCCCGC69550.0144.422018
CTTACCC70850.0141.980245
TTAGGCT94350.0140.54043
CAGGTCA65650.0140.41993
TCTTACC73350.0137.744374
TACCCCG74350.0135.098197
GTCAATT68700.0134.185846
GCTGTGG100200.0132.335197
CACGGTC453.5173252E-6131.10857
CCCCGCC78800.0127.094579
AAATCTT82800.0122.557951
TTACCCC86150.0117.107366
AATTTCA79400.0116.474399
Produced by FastQC (version 0.11.5)