FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1595883
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT343592.1529773799207086No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG300341.881967537720497No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT297391.8634824733392108No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA232061.4541166238377126No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG95250.5968482651923731No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT38040.23836333866580445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT46600.0197.835518
AGGCTGT79850.0174.194055
ATTAGGC80850.0173.123292
GCAGGTC59450.0166.471532
ATCTTAC66550.0166.21973
ACCCCGC70800.0154.381448
CTTACCC72050.0152.721925
TCTTACC72800.0152.562074
CAGGTCA66100.0150.839313
AGTCTTT62400.0147.978949
GTCAATT67400.0147.939246
TACCCCG75500.0145.16177
TTAGGCT97000.0144.147113
GCCATAC64350.0142.555481
CCCCGCC80050.0136.910779
GCTGTGG101250.0135.341457
AATTTCA73250.0135.323069
AAATCTT83350.0133.415991
AAGGTCC1550.0133.18962295
TTACCCC85400.0128.675236
Produced by FastQC (version 0.11.5)