FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374857
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT88652.3649018158924604No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG75472.013301072142177No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT47081.2559456005890246No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA45641.2175309518029542No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)