FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374857
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG83102.2168453570294804No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT68101.8166927655079137No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA51671.378392293594624No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT39861.0633388198699771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT10300.0213.373788
GCAGGTC13700.0202.408752
CAGGTCA15100.0183.642383
GTCAATT16150.0172.616096
AATTTCA16550.0167.552899
AGTCTTT14350.0153.15339
GGCAGGT19550.0144.104861
AGGTCAA19350.0143.307484
GCCATAC15550.0143.23151
GGTCAAT20350.0137.7155
ATTAGGC13800.0134.67392
TCAATTT20750.0134.34947
TCTTACC11550.0125.1515054
ACCCCGC12050.0118.734448
ATCTTAC12650.0115.4347843
CAATTTC25000.0112.18
AGGCTGT16450.0110.288755
CTAGTCT20500.0107.207317
TACCCCG13850.0103.3032467
ATACTAG22300.099.215254
Produced by FastQC (version 0.11.5)