FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360007
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT148404.12214206945976No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA108733.020219051296225No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT103262.868277561269642No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG80862.2460674375776026No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT13970.3880480101775799No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG11200.31110506184601966No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA4970.13805287119417123No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT4050.1124978125425339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT17850.0242.114848
ATTAGGC23700.0208.491582
AGGCTGT24700.0200.050615
GATCCTT150.002848887196.66667295
CGCTGGC150.002848887196.666674
TCTACGA150.002848887196.66667295
AGTCTTT22400.0192.935269
GCCATAC22450.0191.848561
ATCTTAC16250.0186.076923
CTTACCC16250.0185.169225
ACCCCGC16800.0179.107138
GCAGGTC14550.0176.391742
TCTTACC17300.0173.930634
GCTGTGG29300.0169.146767
TTAGGCT29350.0168.356053
CAGGTCA15550.0165.048223
TACCCCG18350.0163.97827
CCCCGCC18550.0162.210249
TTACCCC18600.0161.774196
TAGGCTG32550.0152.258064
Produced by FastQC (version 0.11.5)