FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360007
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT129673.6018744079976224No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA115933.220215162482952No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT90662.51828436669287No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG89182.4771740549489314No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT14520.4033254908932326No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG12540.3483265603168827No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA4160.11555330868566445No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAACAGCGGTGGGCCTAGCCCTACT3750.10416464124308694No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTCT108.462617E-4295.08
TAGTCTT17600.0249.744318
GCCATAC20650.0214.285721
ATTAGGC24300.0211.234572
AGTCTTT21450.0204.918439
AGGCTGT25600.0198.77935
AAGGTCC457.8216544E-11196.66667295
ACCCCGC15100.0186.572868
GCAGGTC16700.0182.829352
TCTTACC15650.0179.073494
TTAGGCT29050.0176.695343
ATCTTAC16100.0174.068313
CAGGTCA17800.0171.530883
CTTACCC16450.0171.26145
GCTGTGG30150.0167.802677
TACCCCG16850.0167.195857
GTCAATT17700.0166.666676
AATTTCA18200.0162.087929
CCCCGCC17900.0158.21239
CCATACT28000.0158.03572
Produced by FastQC (version 0.11.5)