FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569116
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA76201.3389186035887235No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG69921.2285720310094954No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT69701.2247063867471657No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT66001.1596932786988943No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG7990.1403931711636995No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6930.12176779426338391No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG5790.10173672854040303No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCT151.5189675E-5245.05
TCGTACC301.1774064E-6163.333334
TACACGC150.0049663074163.33333245
GTACCTC251.1644203E-4147.06
TACCTCG251.1644203E-4147.07
GTCAATT16800.0136.354166
AGGCTGT23750.0132.04215
TAGTCTT14650.0129.607518
ATCTTAC13500.0128.851853
CAGGTCA17900.0128.659213
ATTAGGC28250.0126.185852
GCAGGTC18450.0123.495932
CTTACCC14200.0122.499995
GGCTGTA950.0116.052636
TTAGGCT30200.0116.009943
AATTTCA19900.0113.881919
AGTCTTT18400.0104.524469
GCTGTGG29600.0103.048997
TCTTACC17000.0102.3235244
GCCATAC19500.0101.769231
Produced by FastQC (version 0.11.5)