FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513775
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT66411.2925891684102964No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG58951.147389421439346No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT57031.110018977178726No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA48990.9535302418373801No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG12150.23648484258673547No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG7380.1436426451267578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC201.5609112E-7245.000024
CGTACCC352.5359695E-6140.05
AGGCTGT22350.0131.543625
GTCAATT13000.0123.4423146
ATTAGGC24650.0122.251522
CAGGTCA13600.0118.897063
ATCTTAC15100.0116.82123
GCAGGTC13750.0115.818192
TAGTCTT14200.0110.422548
TTAGGCT27050.0109.5933463
CTTACCC16650.0107.417425
GCTGTGG28200.099.476957
GTACCCC501.493578E-598.06
ACCCCGC19400.094.716498
AGTCTTT16650.094.174179
AGGTCAA18150.092.465574
TAGGCTG33000.089.0909044
GGTCAAT18700.088.435835
GGGTTAT11750.087.57447245
TCTTACC20600.086.820394
Produced by FastQC (version 0.11.5)