FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513775
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG71141.3846528149481776No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT61121.1896258089630674No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA56031.0905552041263198No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT53341.0381976546153473No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG8050.15668337307187No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG8040.1564887353413459No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6050.11775582696705755No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACA150.004966168163.33333245
GTCAATT16100.0131.630456
ATCTTAC13350.0131.217223
GCAGGTC16250.0129.661542
CAGGTCA16500.0129.181813
AGGCTGT26000.0122.55
TCGTACC302.4065448E-4122.499994
CTTACCC14300.0118.216785
TAGTCTT12600.0117.6388858
TTAGGCT29600.0115.0506743
ATTAGGC30000.0114.741672
GGCTGTA1250.0107.7999956
TCTTACC16550.0105.1057364
AATTTCA19700.0104.467019
GGCAGGT23750.099.031581
AGGTCAA22200.098.220724
ACCCCGC17350.098.141218
TCAATTT21400.097.885517
GGTCAAT22800.094.56145
GCTGTGG30950.093.408737
Produced by FastQC (version 0.11.5)