FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512703
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG66961.3060192743167098No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT63911.2465306424967282No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT59411.158760529975444No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA54751.0678697023422918No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG10890.2124036723015079No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG5870.11449123566665302No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT5560.10844485013740898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC500.0245.04
GTACCCC550.0222.727286
CGTACCC600.0204.166675
TCAATGT150.004966164163.333337
TTACCCG150.004966164163.333336
GCCGTGG251.1643536E-4147.07
GCAGGTC14950.0135.200672
AGGCTGT19900.0131.733675
TAGTCTT13500.0127.9444358
CAGGTCA16100.0125.543473
GTCAATT15650.0125.2396246
GGCCGTG302.4065412E-4122.56
ATCTTAC13200.0111.363643
ATTAGGC24100.0110.3008352
AATTTCA18750.0103.879999
TGAGCAT6900.0102.97101245
CTTACCC14300.0101.940565
GGCAGGT21800.0100.022941
TTAGGCT26500.099.849063
AGTCTTT17950.096.908079
Produced by FastQC (version 0.11.5)