FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259788
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT435143.4540732250188126No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG266112.112339536493442No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT257492.0439153254357083No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA163311.2963292236471533No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT125290.9945324133901895No Hit
GGCAGGTCAATTTCACTGGTAGTAATATAATTGTTGGGACGATTAGTTTT57190.45396527034707423No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG47860.37990519039711446No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA23200.18415796943612733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT39550.0209.584498
ATCTTAC72150.0189.297093
CTTACCC73100.0186.635245
ACCCCGC73000.0185.880688
TCTTACC76700.0178.25994
TACCCCG77850.0174.679357
AGGCTGT64200.0173.223045
CCCCGCC80100.0169.220239
ATTAGGC67300.0166.997212
AGTCTTT50950.0162.979699
AAATCTT85450.0160.524061
TTACCCC85700.0159.02326
GCCATAC55550.0149.749151
GCAGGTC66250.0149.607272
GACCGAC200.0067388266147.49228295
CACCGGG301.1493432E-4147.49228295
CAGGTCA70050.0141.912633
TTAGGCT79650.0140.54823
GCTGTGG79500.0140.071277
GTCAATT72350.0138.012826
Produced by FastQC (version 0.11.5)