FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293442
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT243011.8787854422540786No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA221701.714031243766632No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT191581.4811642114605834No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG184061.4230247664758064No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG22360.17287207311962965No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCC150.002849845196.66418295
TAGTCTT36450.0192.61768
AGGCTGT56000.0155.663225
AGTCTTT47700.0147.188929
ATTAGGC60450.0145.180152
GCAGGTC40600.0141.685412
CAGGTCA41450.0138.77993
GCCATAC53100.0134.164981
ATCTTAC41650.0131.38493
GTCAATT43800.0131.333976
TTAGGCT69750.0125.399853
CTTACCC44750.0122.283385
GCTGTGG71000.0122.15347
AATTTCA52250.0110.65899
TACCCCG49650.0109.918067
ACCCCGC49600.0109.434118
CTGTGGG80100.0109.19658
TCTTACC52250.0105.013044
TCTCGTT2250.0104.88757295
CCCCGCC52400.0103.8679669
Produced by FastQC (version 0.11.5)