FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293442
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT245471.8978044628209074No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA226401.7503683968821175No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG189061.4616813123433443No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT188801.4596711719582325No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG22900.17704698007332373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT39700.0196.920188
AGTCTTT48450.0161.661139
AGGCTGT55000.0157.15925
ATTAGGC57450.0149.926122
ACGGTCC503.3574906E-8147.45876295
GCCATAC54650.0143.028781
ATCTTAC41200.0141.054553
TTAGGCT62800.0137.868973
GCAGGTC41200.0136.389922
CAGGTCA42100.0133.134193
GCTGTGG66900.0128.768137
GTCAATT43950.0127.204366
CTTACCC46350.0124.113715
ACCCCGC49700.0115.451118
TACCCCG50750.0113.357497
TCTTACC51100.0113.154044
CTGTGGG76700.0112.5033348
AATTTCA52250.0108.687439
TAGGCTG81550.0106.17424
CCCCGCC55150.0103.7746059
Produced by FastQC (version 0.11.5)