FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193684
Sequences flagged as poor quality0
Sequence length301
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT263822.210132664926396No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG218831.8332322457199728No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA199251.6692022344272017No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT183021.533236601981764No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG28070.23515436246108684No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT42000.0216.327568
GCAGGTC41650.0176.357832
AGTCTTT55550.0164.091039
GCCATAC56750.0161.660871
AGGCTGT57950.0155.259025
ATTAGGC58200.0154.338672
CAGGTCA47950.0153.494343
GTCAATT47950.0151.648716
AATTTCA53600.0136.213719
GCGGTCG453.5170779E-6131.10763
GGCAGGT59250.0124.7181851
TTAGGCT73300.0123.148153
ATCTTAC44900.0121.8731463
ACCCCGC46150.0118.891748
CGGAAGG1001.8189894E-12117.99685295
CGCAATT505.9394133E-6117.99685295
TCTTACC46600.0117.743644
GCTGTGG77400.0117.005927
CTTACCC47000.0116.741565
TCAATTT64650.0112.932027
Produced by FastQC (version 0.11.5)