FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193684
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT259912.1773769272269714No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG228821.9169227366706767No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA210221.761102603369066No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT184841.5484835182510615No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG26730.2239286109221536No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCTT41450.0210.678828
GCAGGTC42750.0174.237882
AGTCTTT53200.0164.424599
ATTAGGC57650.0163.233282
GCCATAC53750.0161.891681
AGGCTGT57950.0159.079395
CAGGTCA47250.0157.331633
GTCAATT48700.0151.145546
AATTTCA53150.0138.490839
TTAGGCT68500.0136.947433
ATCTTAC44300.0134.180273
ACCCCGC46950.0125.360588
GGCAGGT61700.0122.3871151
GCTGTGG75200.0121.225617
TCTTACC50000.0119.178714
CTTACCC50400.0117.6475455
AGGTCAA63000.0117.296354
TCAATTT64350.0115.303687
GGTCAAT64350.0114.606385
TACCCCG53450.0110.943557
Produced by FastQC (version 0.11.5)