FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545597
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT72471.3282697668792167No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG69531.2743838400870973No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT66281.214816063871319No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA60851.1152920562246493No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG13820.2533005130160173No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG6270.1149199867301323No Hit
AATTAGGCTGTGGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGG6210.11382027393845641No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT6070.11125427742454597No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT352.5361114E-6140.0245
AGGCTGT22750.0126.538465
TAGTCTT13450.0125.687748
GCAGGTC15200.0120.888152
ATCTTAC17100.0118.918123
GTCAATT15400.0118.522726
ATTAGGC25800.0116.3275152
CAGGTCA15850.0115.9305953
CTTACCC18100.0110.994485
TTAGGCT27050.0110.046213
GGGTTAT13650.0103.20513245
GCTGTGG29000.097.577587
AGTCTTT17100.097.4268959
ACCCCGC20950.097.064448
GAAGGTC654.8464244E-794.230772
TCTTACC21600.093.5763854
CGTACCC407.55668E-491.874995
TCGTACC407.55668E-491.874994
GCCATAC19500.088.576931
GGCAGGT22350.087.695751
Produced by FastQC (version 0.11.5)