FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545597
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG78371.4364081913940143No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT67231.232228183072854No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA62771.1504828655582784No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT62481.145167587065178No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG9210.16880591352225177No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG7440.1363643861678125No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG6590.12078512161907048No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC150.004966251163.333332
TCGTACC301.1773609E-6163.333334
GGCTGTA1300.0150.769236
AGGCTGT24050.0133.96055
CAGGTCA17000.0128.985293
GCGTCGG1050.0128.33334245
TAGTCTT14150.0126.395758
ATTAGGC27900.0124.6953352
ATCTTAC14800.0124.15543
GCAGGTC17750.0123.535212
TTAGGCT28100.0123.371893
CGTACCC404.927795E-6122.499995
GTCAATT17450.0121.446986
CTTACCC15650.0116.629395
GTGGAAT1200.0112.2916645
GTACCCC458.849931E-6108.888896
TCTTACC17200.0107.543614
AATTTCA20450.0103.0317849
ACCCCGC17900.0102.6536258
GAAGGTC603.0086085E-7102.0833362
Produced by FastQC (version 0.11.5)