FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370298
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT107692.9081982619403832No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT81852.2103819086249454No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA66891.8063829672317973No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG64711.7475114637400146No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT18430.49770725199704025No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA5740.15501028901047265No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG4160.11234195161734603No Hit
AATTAGGCTGTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGA4150.1120718988490351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)