FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370298
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT89992.430204862030041No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA78922.1312564475098434No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG70941.9157543383977227No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT65621.7720862656563092No Hit
AATTAGGCTGTGGGTGGTTGGCCATGGGTATGTTGTTAAGAAGAGGAATT19920.5379451144753685No Hit
AATTAGGCTGTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGA5240.14150765059492623No Hit
AATTAGGCTGTGGGTGGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAG4650.1255745372645815No Hit
AATTAGGCTGTGGGTGGTTGTGGGTATAATACTAAGTTGAGATGATATCA4400.1188232180568083No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCC108.462713E-4295.0295
ATCTTAC14900.0213.82553
AAGGTCC900.0213.05556295
TAGTCTT12300.0199.065038
AGGCTGT21250.0185.32945
GGTGCCA255.5534736E-5177.01
ACCCCGC18000.0177.08
TCTTACC18050.0176.50974
AGTCTTT13950.0175.519739
CTTACCC18850.0169.018595
TACCCCG19000.0167.68427
ATTAGGC23450.0167.313432
AAATCTT19150.0166.370761
CCCCGCC20000.0159.39
GCTGTGG25000.0157.537
TTAGGCT25400.0155.049213
TTACCCC20750.0154.2536
TACGCTC200.0067358646147.5295
TCTCGCG301.14845345E-4147.5295
CCGGTCC401.9551808E-6147.5295
Produced by FastQC (version 0.11.5)