FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641984
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT90021.4022156315422192No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA76251.1877243046555677No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG74831.165605373342638No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT62540.9741675805004487No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG12120.1887897517695145No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG7820.12180988934303659No Hit
AATTAGGCTGTGGGTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTT7400.11526767022231083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGT24650.0141.13595
ATCTTAC18050.0130.983383
GTACCCC302.406821E-4122.56
GCTTGGG302.406821E-4122.57
CGTACCC302.406821E-4122.55
TCGTACC302.406821E-4122.54
ATTAGGC29600.0121.6722952
CAGGTCA18550.0120.188683
CTTACCC19750.0119.708865
GTCAATT18650.0117.573726
TAGTCTT13200.0116.003798
GCAGGTC19450.0114.627252
TTAGGCT31300.0113.1070253
GCTGTGG33300.0104.1066067
ACCCCGC23500.0100.6063848
TAGGCTG37000.096.013514
TCTTACC24450.095.695294
AGTCTTT16850.092.329389
TACCCCG25950.090.635837
AAATCTT26450.090.3119051
Produced by FastQC (version 0.11.5)