FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005444685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641984
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT83581.3019016050244243No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG82491.2849229887349216No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA75951.1830512909979065No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT60710.9456621971887149No Hit
AATTAGGCTGTGGGTGTGATAGGTGGCACGGAGAATTTTGGATTCTCAGG8320.12959824543913867No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG7700.1199406838799721No Hit
GGCAGGAGTAATCAGAGGTGTTCTTGTGTTGTGATAAGGGTGGAGAGGTT6520.10156016349317117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)