FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663971
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA135672.0433121326082015No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT133652.0128891171451766No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT108051.627330109296942No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG95621.4401231379081316No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC100.0014760614245.000024
AATTAGC100.0014760614245.000021
TCTGACC151.5190812E-5245.04
GTACCCC150.0049664928163.333336
TTACCCG150.0049664928163.333336
TAGTCTT22250.0152.505628
AGGCTGT27350.0141.087755
GTCAATT19850.0133.299746
GCAGGTC19900.0132.349262
ATCTTAC18950.0131.873353
CAGGTCA20300.0130.344833
AGTCTTT26600.0128.486859
CTTACCC20300.0123.7068945
ATTAGGC31850.0120.7692262
TTAGGCT33100.0115.838363
GCTGTGG34550.0111.685967
GCCATAC30600.0110.8905261
AATTTCA25800.0103.032949
TCTTACC25400.098.3858264
ACCCCGC25950.098.188828
Produced by FastQC (version 0.11.5)