FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663971
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA135972.0478304022314227No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT126481.904902473150183No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT102941.5503689167147359No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG100731.5170843304903376No Hit
AATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTA6660.10030558563551722No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC100.0014760614245.000025
TCGTACC100.0014760614245.000024
TAGTCTT22350.0156.756168
AGGCTGT26900.0137.983285
ATCTTAC18000.0136.791673
AGTCTTT26200.0133.721399
GTCAATT19800.0129.924266
CAGGTCA20400.0126.102943
CTTACCC21200.0119.033035
GCAGGTC21850.0117.734552
ATTAGGC32200.0117.5543442
GCCATAC29350.0117.282791
GCGTCGG2200.0116.931816245
TTAGGCT32800.0115.7774353
GCTGTGG33500.0110.7985157
TTACCCG354.4444832E-4105.06
AATTTCA25150.0102.286289
GACGTGG9950.0102.185936245
ACCCCGC25350.0100.5128258
GACTGAG759.920768E-998.0245
Produced by FastQC (version 0.11.5)