FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642946
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT71001.1042918067769298No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT65411.0173482687504083No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG64661.0056832144534689No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA55230.8590145984266174No Hit
AATTAGGCTGTGGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG11560.1797973702301593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCC550.0200.454545
TCGTACC650.0169.615394
GTACCCC801.8189894E-12122.500016
AGGCTGT26000.0121.086545
ATTAGGC27700.0118.962092
GTGGAAT1650.0118.787885
TAGTCTT13900.0118.093538
GCAGGTC14600.0109.075332
GTCAATT14800.0107.601356
CAGGTCA15050.0105.813963
GGCCGTG354.444418E-4105.06
TTAGGCT30950.0104.095323
ATCTTAC15400.095.4545443
AGTCTTT18050.090.9418269
GCTGTGG33700.088.694367
CTTACCC17700.084.435035
GGGTTAT13300.083.815796245
TAGGCTG38600.083.147674
ACCCCGC20550.079.878348
GCCATAC22000.078.511371
Produced by FastQC (version 0.11.5)