FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005444689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005444689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642946
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG80371.2500272184600263No Hit
GCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTACT72681.1304215284020742No Hit
AAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGT61260.9528016349740102No Hit
AATTAGGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAA60750.9448693980520916No Hit
AATTAGGCTGTAGGTAGAAGTAGAGGTTAAGGAGGGTGATGGTGGCTATG6820.10607422707350228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC500.0196.04
TCGATCC150.004966456163.33333245
ACACTCG150.004966456163.33333245
CGTACCC601.2732926E-11142.916675
CAGGTCA18050.0124.196683
GTCAATT17950.0123.523686
GCAGGTC18300.0122.52
AGGCTGT24350.0122.248465
ATTAGGC28600.0118.21682
TAGTCTT15250.0117.278698
TTAGGCT30700.0106.539093
ATCTTAC13900.0103.9928053
CTTACCC13900.0102.230225
GTGGAAT1700.0100.8823555
AATTTCA23000.098.09
AGGTCAA24400.090.87094
AGTCTTT19900.090.4899449
TCTTACC15850.090.425874
GGCAGGT26400.089.090911
GCTGTGG32050.088.291737
Produced by FastQC (version 0.11.5)